Background: Excess high fructose corn syrup (HFCS) intake may contribute to high prevalence of obesity, diabetes, non-alcoholic fatty liver disease, dyslipidemia and hypertension and metabolic syndrome in the world. The influence HFCS diet and kefir-treatment on mouth development process; especially mandible has been poorly documented. Aim: In this study, we investigated the effect of dietary HFCS on the expression of mandible of antioxidant enzymes in association with metabolic parameters in the young male rats. Methods: HFCS was given to the rats as 20% solution in drinking water for eight weeks. Plasma glucose and triglyceride levels were determined by standard enzymatic techniques. Plasma levels of insulin, total testosterone, and estradiol were measured by commercial ELISA kits. Gene expression levels of CAT, GPx, SOD-1, and SOD-2 in the mandibles were established by real-time PCR. Results: HFCS diet elevated plasma glucose, insulin, triglyceride, and estradiol levels, but did not change testosterone expression in rats. Dietary HFCS reduced the levels of CAT, GPx, SOD-1, and SOD-2 mRNAs in the mandibles of rats. Kefir supplementation normalized extremely all these disorders in the HFCS-fed rats. Conclusion: These changes could be responsible for the development of mandible disorders and intake of kefir could have beneficial effects on mouth formation in childhood.
Anahtar Kelimeler: HFCS, Kefir, Mandible
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