Scopoletin is a natural type of coumarin with antioxidant and anti-inflammatory properties. This study aimed to investigate the wound-healing effect of Scopoletin by looking at cell viability, oxidative stress parameters, and changes in FN1 gene levels in fibroblast cells. For this purpose, cells were seeded in 96-well and 6-well plates. Wells (except for the control group) were drawn with a 100 µL pipette tip for the wound line. Then, 50-100-200 µM doses of Scopoletin were applied to the wells except for the control and wound groups, respectively. After 48 hours, cell viability was determined by MTT analysis. LDH, TOS, TAC, and 8-OHdG tests were performed to examine the oxidative stress parameters of the samples from which cell fluid was taken. Then, the expression level of the FN1 gene was examined for molecular analysis. In the statistical method, a one-way ANOVA test was applied. The p<0.05 level was considered statistically significant. According to the findings, MTT levels were significantly decreased in the wound group (57%) compared to the control group. However, treatment with Scopoletin (50-100-200 µM) significantly increased cell viability by 76.99%, 86.90%, and 97.43%, respectively. The FN1 gene expression level was up-regulated in the Scopoletin groups compared to the control group. The most important finding of this study is that Scopoletin suppresses oxidative stress by decreasing 8-OHdG, TOS, and LDH levels and increases cell proliferation by increasing TAC levels and activating antioxidants. Data from this study showed that Scopoletin could be used as an alternative option for enhancing cell viability and modulation of the tissue repair process.
Anahtar Kelimeler: Fibroblast, FN1, LDH, Scopoletin, TAC, 8-OHdG
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