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WOUND HEALING FEATURE OF COENZYME Q10 IN HUMAN DERMAL FIBROBLASTS BY REGULATING THE EXPRESSION OF FN1 GENE
 
Aim:Wound healing is a complex process that consists of several phases such as the accumulation of radical substances, to proliferation, formation of fibrous tissues and collagen. Fibronectin 1 (FN1) has a main role in cellular growth and tissue repair and is expressed highly in human dermal fibroblasts. Coenzyme Q10 has been shown to possess significant anti-inflammatory and anti-oxidant effects. Coenzyme Q10 plays a crucial role in the wound healing process, however detail mechanism still unclear. In this study, we investigated the effects of Coenzyme Q10 on oxidative damage and FN1 gene in fibroblast cells. Methods: Fibroblast cells were cultured in proculture medium. 100 L pipette tip was used to create of wound model. 15M, 30M, and 60M Coenzyme Q10 were added into the medium for 48h. The proliferation-inducing effect on cell viability was determined by using MTT and LDH tests. Oxidative stress parameters including total antioxidant status (TAS), total oxidant status (TOS), and 8-hydroxy-2' -deoxyguanosine (8-OHdG) were eveluated. Also, FN1 expression levels were determined RT-PCR. Results: Coenzyme Q10 demonstrated important proliferation-inducing effect on cell viability. Coenzyme Q10 showed a wound-healing effect by ameliorating oxidative stress via decreasing TOS and 8-OHDG levels and increasing TAS levels. Coenzyme Q10 also elevated the FN1 gene in fibroblast cells. Conclusion: Coenzyme Q10 has shown wound-healing roles with antioxidant properties, as well as cellular growth and repair

Anahtar Kelimeler: Coenzyme Q10, wound healing, oxidative stress, FN1 gene